Tuesday, December 15, 2015

Fixable Yellow LIVE/DEAD stain showing a very strange result

Today an experiment which has been run multiple times on our Astrios was brought and looked REALLY weird.  The culprit seems to be the Fixable Yellow LIVE/DEAD stain, though most likely due to some kind of mishandling.


Update:  Here are some better pictures, including pictures showing the difference between Dec. 15's trial of this and a successful (and normal) trial on Nov. 16.

Monday, June 15, 2015

a bad sorting experience with the Beckman Coulter Astrios

The other day I was running a typical cell sort on the Astrios from murine spleen cells, and I noticed a lot of liquid building up next to my collection tube.  Upon further investigation (placing a slide across the open area) I saw THIS?!!!?  WHY?????

Deflection looks fine to me (see below).